Optimizing Image Acquisition and Data Analysis with the Zeiss LSM510 Meta System

Zifu Wang, UC Irvine

Location: 4248 McGaugh Hall

This workshop includes a 45-minute presentation from 2 - 2:45 p.m., followed by a discussion session from 2:45 – 3:30 p.m.

Due to the limitation of space, please reserve your seat through registration at http://dbc.bio.uci.edu/registration/

Workshop Abstract:

  • Optimizing the acquisition of your confocal image is the key to the successful interpretation and understanding of the underlying biological mechanisms and to obtaining good publication quality figures. For this purpose, it is essential to avoid signal saturation and to set a proper background level. In this workshop we will introduce the concept of the dynamic range of an imaging system. You will learn how to use the dynamic range as an objective and scientific criteria to optimize your image acquisition.
  • Co-localization describes the presence of two or more types of molecules at the same physical location, which is usually evaluated based on the colors in a merged image. In this seminar, you will learn how to use the "line profile" to objectively evaluate the co-localization in your images. In addition, you will learn how to conduct reliable quantitative analysis of the degree of the co-localization with various co-localization coefficients.
  • Fluorescence intensity is proportionally related to the expression level of stained proteins. We will demonstrate how to conduct a quantitative image analysis of changes in the level of proteins in different domains of cells and tissues